摘要：

Estrogen receptors (ER) were analyzed in 63 human breast cancers by immunocytochemical assay, and by enzyme-immunoassay, usingmonoclonal antibodies against human ER protein, and also by the dextrancoated characoal method. The specific staining wasobserved only in the nucleus of cancer cells by the immunocytochemical assay, and the presence of nuclear staining by thisassay was closely associated with the estimation of the cytosolic ER content by both the dextran-coated charcoal method andthe enzyme-immunoassay. In the latter, the cytosolic ER content corres-pondingly increased with the nuclear ER content. Boththe cytosolic and nuclear ER contents correlated well with those obtained by the dextrancoated charcoal method. Furthermore,the cytosolic ER contents obtained by the enzyme-immunoassay and the dextran-coated charcoal method correlated significantlywith the age of the patient, but not with the nuclear ER contents.

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