摘要：

Much of what we know about the molecular level structure-function relationships in P450s is based on studies with the camphor monooxygenase system from Pseudomonasputida. Given that P450cam was the first P450 to be purified in sufficient quantities for structure-function relationships, it is not too surprising that P450cam was the first P450 crystal structure to be solved. The high-resolution structure of P450cam was published in 19871 and remained the paradigm for P450 structure-function studies until the P450BM3 structure was solved in 19932. Since then the rate at which new P450 structures are being solved has increased dramatically and at present there are structures for 20 unique P450s on deposit in the Protein Data Bank with others waiting in the wings. Some of the new advances that have been made since the last edition of this book are the structures of other redox components of P450 monooxygenase systems, of the first electron transfer complex, of new substrate complexes, of the first P450s from thermophilic organisms, and of the first membrane-bound P450. hi the present chapter, we summarize some of these more important recent findings with full recognition that the P450 structural field is moving much more quickly than in the time frame of previous editions of this book. As a result, the current chapter must be considered a snapshot in time on where we now stand in P450 crystallography.

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