CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme.
摘要：
We have cloned a novel apoptotic gene from human Jurkat T-lymphocytes. The new gene encodes a 32-kDa putative cysteine protease (CPP32) with significant homology to Caenorhabditis elegans cell death protein Ced-3, mammalian interleukin-1 beta-converting enzyme (ICE), and the product of the mouse nedd2 gene. The CPP32 transcript is highly expressed and most abundant in cell lines of lymphocytic origin. Overexpression of CPP32 or ICE in Sf9 insect cells resulted in apoptosis. In addition, coexpression of recombinant p20 and p11 derived from the parental full-length CPP32 sequence resulted in apoptosis in Sf9 cells. Our data suggest that similar to ICE, CPP32 is made of two subunits, p20 and p11, which form the active CPP32 complex. The apoptotic activity of CPP32 and its high expression in lymphocytes suggest that CPP32 is an important mediator of apoptosis in the immune system.
展开
关键词：
Cell Line Animals Humans Spodoptera Caenorhabditis elegans Cysteine Endopeptidases Caspases Caspase 1 Helminth Proteins Caenorhabditis elegans Proteins
DOI：
10.1093/nar/22.24.5512
被引量：
年份：
1994
Elsevier
BMJ
Semantic Scholar
万方医学
Europe PMC
查看更多
NCBI
NCBI
Oxford Univ Press
NRC Research Press
AACR
JBC
ResearchGate
EBSCO
diabetes.diabetesjournals.org
学术范
(全网免费下载)
biolreprod.org
mcb.asm.org
molbiolcell.org
stroke.ahajournals.org
ajcn.nutrition.org
ajpgi.physiology.org
molpharm.aspetjournals.org
jem.rupress.org
frontiersin.org
jacionline.org
mendeley.com
jasn.asnjournals.org
jimmunol.org
pharmgkb.org
journalofsurgicalresearch.com
fasebj.org
deepdyve.com
ar.iiarjournals.org
dev.biologists.org
ejso.com
bloodjournal.org
mysciencework.com
jneurosci.org
学术范
www.socolar.com
emboj.embopress.org
pubfacts.com
学术范
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
来源期刊
引用走势
站内活动
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
