Taxol-induced Apoptosis and Phosphorylation of Bcl-2 Protein Involves c-Raf-1 and Represents a Novel c-Raf-1 Signal Transduction Pathway
摘要：
c-Raf-1 (Raf-1) is a central component of signal transduction pathways stimulated by various growth factors, protein kinase C, and other protein kinases. Raf-1 activation is thought to be initiated at the plasma membrane after its recruitment by Ras. Raf-1 activation is associated primarily with proliferation and cell survival, but it has also been implicated in apoptosis. Raf-1 has also been shown to form complexes with both R-Ras and Bcl-2, raising the possibility that this component of cellular Raf-1 plays a role in apoptosis. Recently, taxol was reported to induce Bcl-2 phosphorylation and inactivation. We have previously demonstrated Raf-1 activation following taxol in MCF7 cells. We now present evidence that taxol fails to stimulate either apoptosis or phosphorylation of Bel-2 in the absence of Raf-1. Moreover, Raf-1 activation by taxol coincided with Bel-2 phosphorylation, showing similar dose and time dependence. Thus, our data support a role for a distinct subcellular component of Raf-1, which is taxol but not phorbol myristate acetate sensitive, in mediating an apoptotic pathway involving Bc1-2.
展开
关键词：
pancreaticobiliary maljunction K-ras oncogene polymerase chain reaction single strand conformation polymorphism
DOI：
doi:10.1002/(SICI)1097-0142(19960415)77:8+<1752::AID-CNCR27>3.0.
被引量：
年份：
1996
BMJ
万方医学
NCBI
NCBI
Europe PMC
查看更多
Oxford Univ Press
AACR
(全网免费下载)
AACR
(全网免费下载)
JBC
AACR
ResearchGate
(全网免费下载)
ResearchGate
EBSCO
mcb.asm.org
molbiolcell.org
molpharm.aspetjournals.org
jem.rupress.org
deepdyve.com
jimmunol.org
ajpcell.physiology.org
jn.nutrition.org
clinical-lung-cancer.com
scienceopen.com
ar.iiarjournals.org
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
