Direct electrochemistry and electrocatalytic properties of hemoglobin immobilized on a carbon ionic liquid electrode modified with mesoporous molecular sieve MCM-41
摘要：
The direct electron transfer and electrocatalysis of hemoglobin (Hb) entrapped in the MCM-41 modified carbon ionic liquid electrode (CILE) were investigated by using cyclic voltammetry in 0.10 M pH 7.0 phosphate buffer solution (PBS). Due to its uniform pore structure, high surface areas and good biocompatibility, the mesoporous silica sieve MCM-41 provided a suitable matrix for immobilization of biomolecule. The MCM-41 modified CILE showed significant promotion to the direct electron transfer of Hb, which exhibited a pair of well defined and quasi-reversible peaks for heme Fe(III)/Fe(II) with a formal potential of 0.284 V (vs. Ag/AgCl). Additionally, the Hb immobilized on the MCM-41 modified carbon ionic liquid electrode showed excellent electrocatalytic activity toward H 2O 2. The electrocatalytic current values were linear with increasing concentration of H 2O 2 in a wide range of 5–310 μM and the corresponding detection limit was calculated to be 5 × 10 8 M (S/N = 3). The surface coverage of Hb immobilized on the MCM-41 modified carbon ionic liquid electrode was about 2.54 × 10 9 mol cm 2. The Michaelis–Menten constant K m app of 214 μM indicated that the Hb immobilized on the modified electrode showed high affinity to H 2O 2. The proposed electrode had high stability and good reproducibility due to the protection effect of MCM-41 and ionic liquid, and it would have wide potential applications in direct electrochemistry, biosensors and biocatalysis.
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DOI：
10.1016/j.colsurfb.2010.04.001
被引量：
年份：
2010
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