A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity
摘要:
A method is described for preparing /sup 32/P-labeled ATP of high specific activity by an excbange reaction. The method is simple and requires only substrates and enzymes that are available commercially. Hydrolysis of the labeled ATP with heavy meromyosin indicates that 98 to 99% of the /sup 32/P is in the 1 -phosphate group. (auth)
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关键词:
biology and medicine adenine atp biochemistry chemical reactions economics efficiency enzymes hydrolysis labelled compounds
DOI:
10.1042/bj0900147
被引量:
年份:
1964
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