Multiple nuclear factors interact with the immunoglobulin enhancer sequences
摘要：
To characterize proteins that bind to the immunoglobulin (Ig) heavy chain and the κ light chain enhancers, an electrophoretic mobility shift assay with end-labeled DNA fragments was used. Three binding proteins have been found. One is NF-A, a factor found in all tested cell types that binds to the octamer sequence found upstream of all Ig variable region gene segments and to the same octamer in the heavy chain enhancer. The second, also ubiquitous, protein binds to a sequence in both the heavy chain and the κ enhancers that was previously shown to be protected from methylation in vivo. Other closely related sites do not compete for this binding, implying a restriction enzyme-like binding specificity. The third protein binds to a sequence in the κ enhancer (and to an identical sequence in the SV40 enhancer) and is restricted in its occurrence to B cells.
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关键词：
Animals Humans DNA-Binding Proteins Binding Sites Protein Binding Methylation Genes, Regulator Immunoglobulin Heavy Chains Immunoglobulin kappa-Chains Immunoglobulin mu-Chains
DOI：
10.1016/0092-8674(86)90346-6
被引量：
年份：
1986
Elsevier
万方医学
dx.doi.org
Cell Press
NCBI
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