摘要：

Objective Using the polar mobile phase,the enantioseparation of clenbuterol and mabuterol on the self-made teicoplanin chairal stationary phase were studied.Methods The mobile phase consisted of methanol : ethanol:acetic acid:triethylamine = 50:50:0.05:0.05(V/V/V)with a flow rate of 1.0 mL/min and column temperature of 25 ℃.The influences of the proportion of acetic acid and triethyamine,ethanol concentration,column temperature and flow rate on the enantioseparation were investigated.The chiral recognition mechanism was also discussed.Results Under the optimal conditions,the separation factor for clenbuterol and mabuterol was 1.37 and 1.51,respectively.Conclusion The result showed that the isomers of clenbuterol and mabuterol could be separated on the self-made teicoplanin chairal stationary phase in the polar mobile phase.

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