Selected ion monitoring method for determination of nicotine, cotinine and deuterium-labeled analogs: Absence of an isotope effect in the clearance of (S)-nicotine-3′,3′-d2 in humans

来自 NCBI

阅读量:

38

作者:

PeytonJacobIIILisaYuMargaretWilsonNealL.Benowitz

展开

摘要:

A method for simultaneous determination of nicotine, its metabolite cotinine, and the stable isotope-labeled analogs nicotine-3′,3′-d<sub>2</sub> and cotinine-4′,4′-d<sub>2</sub> in human plasma has been developed. The method utilizes capillary column gas chromatography with detection by electron impact mass spectrometry and selected ion monitoring. Sensitivity is adequate for determination of nicotine and nicotine-d<sub>2</sub> at concentrations as low as 1 ng ml<sup>−1</sup>, and cotinine and cotinine-d<sub>2</sub> at concentrations as low as 10 ng ml<sup>−1</sup> with good precision and accuracy. The method has been used to compare the elimination kinetics of (S)-nicotine-3′,3′-d<sub>2</sub> with natural nicotine in human subjects. Total clearance of nicotine-3′,3′-d<sub>2</sub> was virtually identical to the total clearance of natural nicotine, which validates the use of the deuterium-labeled analog in quantitative studies

展开

DOI:

10.1002/bms.1200200503

被引量:

370

年份:

1991

通过文献互助平台发起求助,成功后即可免费获取论文全文。

相似文献

参考文献

引证文献

引用走势

2002
被引量:29

辅助模式

0

引用

文献可以批量引用啦~
欢迎点我试用!

引用