Conjugated linoleic acid-enriched butter fat alters mammary gland morphogenesis and reduces cancer risk in rats.

来自 dx.doi.org

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107

作者：

C Ip，S Banni，E Angioni，G Carta，D.E. Bauman

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摘要：

Conjugated linoleic acid (CLA) is a potent cancer preventive agent in animal models. To date, all of the in vivo work with CLA has been done with a commercial free fatty acid preparation containing a mixture of 9,11-, 10,12- and 11,13-isomers, although CLA in food is predominantly (80–90%) the 9,11-isomer present in triacylglycerols. The objective of this study was to determine whether a high CLA butter fat has biological activities similar to those of the mixture of free fatty acid CLA isomers. The following four different endpoints were evaluated in rat mammary gland: ) digitized image analysis of epithelial mass in mammary whole mount; ) terminal end bud (TEB) density; ) proliferative activity of TEB cells as determined by proliferating cell nuclear antigen immunohistochemistry; and ) mammary cancer prevention bioassay in the methylnitrosourea model. It should be noted that TEB cells are the target cells for mammary chemical carcinogenesis. Feeding butter fat CLA to rats during the time of pubescent mammary gland development reduced mammary epithelial mass by 22%, decreased the size of the TEB population by 30%, suppressed the proliferation of TEB cells by 30% and inhibited mammary tumor yield by 53% (< 0.05). Furthermore, all of the above variables responded with the same magnitude of change to both butter fat CLA and the mixture of CLA isomers at the level of CLA (0.8%) present in the diet. Interestingly, there appeared to be some selectivity in the uptake or incorporation of 9,11-CLA over 10,12-CLA in the tissues of rats given the mixture of CLA isomers. Rats consuming the CLA-enriched butter fat also consistently accumulated more total CLA in the mammary gland and other tissues (four- to sixfold increases) compared with those consuming free fatty acid CLA (threefold increases) at the same dietary level of intake. We hypothesize that the availability of vaccenic acid (11–18:1) in butter fat may serve as the precursor for the endogenous synthesis of CLA via the Δ9-desaturase reaction. Further studies will be conducted to investigate other attributes of this novel dairy product.

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关键词：

Epithelium Mammary Glands, Animal Animals Rats Rats, Sprague-Dawley Mammary Neoplasms, Experimental Butter Linoleic Acid Protein Isoforms Organ Size