Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase.
摘要：
A rapid assay for retroviral reverse transcriptase activity released into the culture medium by infected cells was developed. With the assay, 4,000 clonally infected cell lines could be tested in a few hours. We have adapted the assay for use as a screen for the detection of spontaneous viral mutants. Mutants of Moloney murine leukemia virus have been isolated which (i) produce a thermolabile reverse transcriptase, (ii) are temperature sensitive for release of enzyme activity, or (iii) can only productively infect cells already producing gag-related polypeptides. The assay has also been useful for the isolation of nonproducer cells infected with various replication-defective transforming viruses.
展开
DOI：
10.1016/0166-0934(81)90060-4
被引量：
年份：
1981
掌桥科研
Semantic Scholar
(全网免费下载)
Semantic Scholar
万方医学
dx.doi.org
查看更多
BioOne
NCBI
Oxford Univ Press
NCBI
Europe PMC
(全网免费下载)
NCBI
(全网免费下载)
Europe PMC
ResearchGate
ResearchGate
(全网免费下载)
EBSCO
www.socolar.com
core.ac.uk
authors.library.caltech.edu
worldcat.org
(全网免费下载)
jvi.asm.org
(全网免费下载)
core.ac.uk
(全网免费下载)
authors.library.caltech.edu
(全网免费下载)
clinchem.org
journals.asm.org
jvi.asm.org
mysciencework.com
en.zl50.com
SAGE
mendeley.com
emboj.embopress.org
jimmunol.org
Semantic Scholar
(全网免费下载)
NCBI
(全网免费下载)
Europe PMC
(全网免费下载)
ResearchGate
(全网免费下载)
authors.library.caltech.edu
(全网免费下载)
jvi.asm.org
(全网免费下载)
core.ac.uk
(全网免费下载)
worldcat.org
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
