Purified DNAs are transcribed after microinjection into Xenopus oocytes
摘要：
The possibility of using DNA-injected Xenopus laevis oocytes and eggs for studying the control of transcription in eukaryotes has been investigated. When purified DNA of simian virus 40 (SV40) is injected into Xenopus laevis oocytes, tritiated RNA precursors are incorporated into DNase-I-resistant, RNase-A- and alkali-sensitive material that hybridizes specifically to SV40 DNA. This viral transcription continues for at least 5 days and occurs only when the injected DNA is directed to the nucleus of the oocyte. The quantity of SV40-specific RNA produced is roughly proportional to the amount of DNA injected; above 1 ng per oocyte, most of the nonribosomal RNA made in successfully injected oocytes is virus-specific. Transcription also occurs, although at a lower efficiency, after injection of the DNA into unfertilized eggs. The DNAs of adenovirus 5, cloned Drosophila melanogaster histone genes, and even bacteriophage φ X174 replicative form, bacteriophage φ 80plac, and the ColE1 plasmid are also transcribed after injection into oocytes or eggs.
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关键词：
Biochemistry Simian virus 40 Cloned DNA of Drosophila melanogaster histone genes RNA· DNA filter hybridization Eukaryotic transcription system
DOI：
10.1073/pnas.74.4.1502
被引量：
年份：
1977
万方医学
掌桥科研
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