Monoclonal antibodies to receptors for insulin and somatomedin-C.

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作者：

FCJ Kull，S Jacobs，YF Su，ME Svoboda，P. C. Cuatrecasas

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摘要：

Three monoclonal , designated alpha IR-1, alpha IR-2, and alpha IR-3, were prepared by fusing FO with spleen from a immunized with a partially purified preparation of from placenta. These were characterized by their ability to immunoprecipitate solubilized receptors labeled with 125I-or 125I-in the presence or absence of various concentrations of unlabeled or . alpha IR-1 preferentially immunoprecipitates and also less effectively immunoprecipitates receptors, while alpha IR-2 and alph IR-3 preferentially immunoprecipitate receptors, but may also weakly immunoprecipitate . These three monoclonal , as well as A410, a polyclonal , were used to immunoprecipitate and receptors from solubilized lymphoid (IM-9) and placenta that had been 125I-labeled with . Analysis of the immunoprecipitates by -gel electrophoresis indicates that both receptors are composed of alpha and beta subunits. The beta subunit of the (immunoprecipitated by alpha IR-1 and A410) has a slightly more rapid mobility than the corresponding subunit of the receptor (immunoprecipitated by alpha IR-2 and alpha IR-3). Interestingly, the alpha subunit of the placenta receptor has a slightly faster mobility than its counterpart from IM-9 . Immunoprecipitation of receptor that had been reduced and denatured to generate isolated subunits indicates that alpha IR-2 and alpha IR-3 interact with the alpha subunit of the receptor while A410 interacts with both subunits of the . alpha IR-1 failed to react with reduced and denatured receptors.

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DOI：

10.1016/S0021-9258(18)32449-9

被引量：

1249

年份：

1983