摘要：

Treatment of intact human erythrocytes with tritiated trinitrobenzenesulfonic acid (TNBS) and picryl chloride (PC) resulted in labeling of their membrane components. Separation of these components by polyacrylamide gel electrophoresis in 1% SDS yielded three significant results: 1) in the protein region significant TNBS label was confined to a single sharp high peak centering on band 3 of Fairbanks et al. (1), 2) protein components of bands 1 and 2 contained significant amounts of PC label and were free of TNBS label, 3) the low molecular weight lipid components were heavily labeled by both reagents. Chemical separation of the protein and lipid components showed about 70% of the TNBS label and about 53% of the PC label to be in the protein fraction with the balance of the activity appearing in the lipid fraction. The selectivity of these membrane-penetrating reagents for different membrane components may be a function of their differing polarities.

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