Cloning and amplification of alpha and beta mouse globin gene sequences synthesised in vitro.
摘要：
New chimeric Escherichia coli plasmids containing α or β globin gene sequences of the mouse were constructed. Double-stranded DNA, synthesised in vitro in a 2-step reaction from mouse globin mRNA was inserted into E. coli plasmid pCR1, after tailing of the 2 DNAs with dG and dC respectively. Some of the mouse globin plasmids described contain at least 90% of the globin mRNA sequence and therefore contain the entire translated sequence of the globin genes. Some possible uses of these recombinant plasmids are described.
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关键词：
alkaloids rearrangements oxidation, dehydrogenation, ozonolysis hydroxylation, hydroboration, phosphorylation, silylation alkylation, arylation, dealkylation, dearylation, C‐acylation, olefination
DOI：
10.1016/0378-1119(77)90047-6
被引量：
年份：
1977
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