The Nucleotide Sequence of the lac Operator
摘要：
The lac repressor protects the lac operator against digestion with deoxyribonuclease. The protected fragment is double-stranded and about 27 base-pairs long. We determined the sequence of RNA transcription copies of this fragment and present a sequence for 24 base pairs. It is: 5′--T G G A A T T G T G A G C G G A T A A C A A T T 3′3′--A C C T T A A C A C T C G C C T A T T G T T A A 5′The sequence has 2-fold symmetry regions; the two longest are separated by one turn of the DNA double helix.
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关键词：
Biochemistry Regulation Protein-nucleic acid interaction DNA-RNA sequencing Oligonucleotide priming
DOI：
10.1073/pnas.70.12.3581
被引量：
年份：
1973
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