Catalase Immobilization on Electrospun Nanofibers: Effects of Porphyrin Pendants and Carbon Nanotubes

来自 ACS

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Carbon nanotube (CNT) has been generally recognized as an electron acceptor, whereas porphyrin can act as an electron donor. In this work, acrylonitrile-based copolymers bearing porphyrin pendants were therefore blended with or without CNTs and then electrospun into nanofibers on which a redox enzyme, catalase, was covalently immobilized. The uniform nanofibers diameters are around 180 nm. Field emission scanning electron microscopy and transmission electron microscopy confirmed the morphologies of the nanofibers and the distribution of CNTs. Rougher surfaces as well as obvious protruded parts induced by the blending of CNTs were observed from the studied nanofibers. Catalase was immobilized onto the nanofiber surface through the activation of carboxyl groups by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (EDC/NHS). Results indicate that both the introduction of porphyrin pendants and CNTs obviously improve the activity and stabilities of the immobilized catalases...

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Bibliography, Experimental/ biochemistry biomembranes carbon nanotubes enzymes field emission electron microscopy molecular biophysics nanobiotechnology nanostructured materials polymer blends polymer fibres rough surfaces scanning electron microscopy transmission electron microscopy/ catalase immobilization electrospun nanofibers porphyrin pendants carbon nanotubes electron acceptor electron donor acrylonitrile-based copolymers redox enzyme field emission scanning electron microscopy transmission electron microscopy rough surface N-(3-dimethylaminopropyl)- N'-ethylcarbodiimide hydrochloride N-hydroxysuccinimide electron transfer fiber membranes C/ A8715D Physical chemistry of biomolecular solutions condensed states A8715K Biomolecular interactions, charge transfer complexes A8720E Natural and artificial biomembranes A8783 Nanotechnology applications in biomedicine/ C/el