摘要：

Native and processed high-(HAMS) is an important source of resistant (RS). The objectives of this work were to use an in vitro procedure to estimate the RS content of native granules from a series of ae-containing HAMS genotypes, and to examine the nature of the resistant (). By the method of Englyst et al (1992), RS for ae V, ae VII, ae , and ae du were estimated to be 66.0, 69.5, 69.5, and 40.6%, respectively. By transmission microscopy, most of the residual granules from ae V, ae VII, and ae showed little evidence of . Partially digested granules had a radial pattern in the interior and an enzyme-resistant layer near the surface. Size and chain-length profile of constituents of were similar to those of the native HAMS (unlike type 3 RS), consistent with complete hydrolysis in susceptible granule regions. Between crossed polarizers, many -stained native and residual HAMS granules had blue centers and pink exteriors, which may be due to a difference in orientation of the -complexes in the exterior. Four granule color types were observed for ae du, differing in enzyme resistance. The high-enzyme resistance of native HAMS granules may result from altered granule organization, which appears to vary among and within granules from ae-containing genotypes.

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