Analysis of a cAMP-responsive activator reveals a two-component mechanism for transcriptional induction via signal-dependent factors.

来自 NCBI

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作者：

T Nakajima，C Uchida，SF Anderson，JD Parvin，M Montminy

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摘要：

We have examined the mechanism by which the cAMP-responsive factor CREB stimulates target gene expression following its phosphorylation at Ser-133. Using an in vitro transcription assay, we found that two signals were required for target gene activation: a phospho(Ser-133)-dependent interaction of CREB with RNA polymerase II via the coactivator CBP and a glutamine-rich domain interaction with TFIID via hTAF(II)130. The adenovirus E1A oncoprotein was found to inhibit phospho(Ser-133) CREB activity by binding to CBP and specifically blocking recruitment of RNA Pol II to the promoter. Our results suggest that the recruitment of CBP-RNA Pol II complexes per se is not sufficient for transcriptional activation and that activator-mediated recruitment of TFIID is additionally required for induction of signal-dependent genes.

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关键词：

DNA-Binding Protein, Cyclic AMP-Responsive Adenovirus E1A Proteins Transcription Factors, TFII DNA结合蛋白质, 环AMP反应性

DOI：

10.1101/gad.11.6.738

被引量：

637

年份：

1997