In vitro antibacterial and cytotoxicity assay of multilayered polyelectrolyte‐functionalized stainless steel

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作者：

Z Shi，KG Neoh，SP Zhong，LYL Yung，ET Kang，W Wang

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摘要：

Infection of implanted materials by bacteria constitutes one of the most serious complications following prosthetic and implant surgery. In the present study, a new strategy for confering stainless steel with antibacterial property via the alternate deposition of quaternized polyethylenimine (PEI) or quaternized polyethylenimine-silver complex and poly(acrylic acid) (PAA) was investigated. The success of the deposition of the polyelectrolyte multilayers (PEM) and its chemical nature was investigated by static water contact angle and X-ray photoelectron spectroscopy (XPS), respectively. The antibacterial activity was assessed using Escherichia coli (E. coli, a gram-negative bacterium) and Staphylococcus aureus (S. aureus, a gram-positive bacterium). The inhibition of E. coli and S aureus growth on the surface of functionalized films was clearly shown using the LIVE/DEAD Baclight bacterial viability kits and fluorescence microscopy. The cytotoxicity of the PEM to mammalian cells, evaluated by the MTT assay, was shown to be minimal and long-term antibacterial efficacy can be maintained. These results indicate new possibilities for the use of such easily built and functionalized architectures for the functionalization of surfaces of implanted medical devices. Â© 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

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关键词：

stainless steel antibacterial surface functionalization polyelectrolyte multilayers cytotoxicity

DOI：

10.1002/jbm.a.30597

被引量：

139

年份：

2006