Clonal deletion of B lymphocytes in a transgenic mouse bearing anti-MHC class I antibody genes

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作者：

DA Nemazee，K Bürki

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摘要：

B lymphocytes can be rendered specifically unresponsive to antigen by experimental manipulation in vivo and in vitro 1–6 , but it remains unclear whether or not natural tolerance involves B-cell tolerance because B cells are controlled by T lymphocytes and in their absence respond poorly to antigen (reviewed in ref. 7). In addition, autoantibody-producing cells can be found in normal mice and their formation is enhanced by B-cell mitogens such as lipopolysaccharides 8–12 . We have studied B-cell tolerance in transgenic mice using genes for IgM anti-H–2 k MHC class I antibody. In H–2 d transgenic mice about 25–50% of the splenic B cells bear membrane immunoglobulin of this specificity and abundant serum IgM encoded by the transgenes is produced. In contrast, H–2 k x H–2 d (H–2-d/k) transgenic mice lack B cells bearing the anti-H–2 k idiotype and contain no detectable serum anti-H–2 k antibody, suggesting that very large numbers of autospecific B cells can be controlled by clonal deletion.

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关键词：

Animals Mice, Inbred BALB C Mice B-Lymphocytes Lymphocyte Depletion Antilymphocyte Serum Immunoglobulin Idiotypes H-2 Antigens Cloning, Molecular Immunologic Capping