Kinetic and mechanistic analysis of nonenzymatic, template...

来自 EBSCO

阅读量:

11

作者:

RohatgiRajatBartelDavidP.

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摘要:

Examines the kinetic and mechanistic characterization of the pyrophosphate-activated, template-directed oligoribonucleotide ligation reaction. Synthesis and purification of RNA; Effects of pH on divalent metal cations; Locations of the strong and weak binding sites; Role of enzymes; Data to suggest that there is a nucleophilic participation in the transition state.

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被引量:

85

年份:

1996

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2004
被引量:12

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