alpha-Ketoacids scavenge H2O2 in vitro and in vivo and reduce menadione-induced DNA injury and cytotoxicity.
摘要：
We demonstrate that alpha-ketoacids reduce and, in some instances, abrogate -induced DNA damage and cytotoxicity in the breast line, MCF7. We confirm that alpha-ketoacids quench the copious amounts of H2O2 generated by while these alpha-ketoacids undergo nonenzymatic oxidative decarboxylation; our data thus support enhanced H2O2 production as an important pathway for -induced DNA damage and cytotoxicity. We also demonstrate that alpha-ketoacids scavenge H2O2 generated by and microsomes when these are exposed to ; additionally, alpha-ketoacids protect oxidant-vulnerable enzymes against functional impairment induced by H2O2. Finally, we provide the first in vivo demonstration that acute elevations in concentrations of alpha-ketoacids in tissues and urine scavenge H2O2. We conclude that enhanced H2O2 production is a major pathway for -induced DNA damage and cytotoxicity and that the diverse alpha-ketoacids present within the must be considered, along with and , as part of the antioxidant defense mechanisms that regulate the ambient levels of H2O2.
展开
DOI：
10.1016/S0065-2776(08)60589-1
被引量：
年份：
1995
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
