摘要：

ObjectiveAn experimental animal model of contact dermatitis (CD) was used to investigate the effects of free and nanoencapsulated clobetasol propionate on the skin and on the oxidative profile of liver tissue.MethodsFemale Wistar rats were divided into six groups, each containing eight rats.The first group, control (C), was sensitized with solid vaseline.Group 2, (CD), was sensitized with 5 NiSO₄.Groups 3 and 4 were sensitized with 5 NiSO₄ and treated with free (FC) and nanoencapsulated (NC) clobetasol (0.42 mg/g), respectively, daily for 5 days.Group 5 was treated with nanoencapsulated clobetasol (0.42 mg/g) on days 1, 3, and 5 (C135) and group 6 received a hydrogel containing empty nanoparticles (NP) daily for 5 days.Thiobarbituric acid reactive substances (TBARS), carbonyl levels, non-protein sulfhydryl groups (NPSH) and catalase activity were measured in liver homogenates.ResultsA significant increase was observed in the levels of TBARS, NPSH, and catalase activity for the groups CD and NP.DiscussionOur results suggest that both NiSO₄ sensitization and NP administration induced oxidation of cellular lipids and activated the antioxidant enzyme catalase to protect from this damage.These results also indicated that daily treatment with the free and nanoencapsulated clobetasol, as well as treatment with the nanoencapsulated clobetasol every other day, were able to prevent these redox alterations and protect against histological damage.

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