Mouse splenic and bone marrow cell populations that express high-affinity Fc epsilon receptors and produce interleukin 4 are highly enriched in basophils.

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摘要：

Splenic and bone marrow cells from normal mice, and from mice that have been polyclonally activated by injection of anti-IgD antibody, contain cells that produce interleukin 4 (IL-4) in response to crosslinkage of Fcεreceptors (FcεR) or FcγR or to ionomycin. Isolated FcεR+cells have recently been shown to contain all of the IL-4-producing capacity of the nonlymphoid compartment of spleen and bone marrow. Here, purified FcεR+cells are shown to be enriched in cells that contain histamine and express alcian blue-positive cytoplasmic granules. By electron microscopy, the vast majority of cytoplasmic granule-containing cells are basophils; they constitute ≈25% and ≈50%, respectively, of FcεR+spleen and bone marrow cells from anti-IgD-injected mice. The FcεR-populations contain cells that form colonies typical of mast cells. The FcεR+populations also contain cells that, upon culture with IL-3, form colonies of alcian blue-positive cells, but (in contrast to colonies derived from FcεR-populations) the colonies are small, and all the cells die within 2-3 weeks. The FcεR+cells synthesize histamine during a 60-hr culture with IL-3, while the FcεR-cells do not. These results indicate that IL-4-producing FcεR+cells are highly enriched in basophils.

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关键词：

Immunology Mast Cell Histamine Immunoglobulin E

DOI：

10.1073/pnas.88.7.2835

被引量：

800

年份：

1991