Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor

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124

作者：

T Yamamoto，S Ikawa，T Akiyama，K Semba，N Nomura，N Miyajima，T Saito，K Toyoshima

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摘要：

A novel v- erb - B -related gene, c- erb - B -2, which has been identified in the human genome 1,2 , maps to human chromosome 17 at q21 (ref. 40) and seems to encode a polypeptide with a kinase domain that is highly homologous with, but distinct from, that of the epidermal growth factor (EGF) receptor 1 . The c- erb - B -2 gene is conserved in vertebrates and it has been suggested1 that the neu gene, detected in a series of rat neuro/glioblastomas 3 , is, in fact, the rat c- erb - B -2 gene. Amplification of the c- erb - B -2 gene in a salivary adenocarcinoma and a gastric cancer cell line MKN-7 suggests that its over-expression is sometimes involved in the neoplastic process. To determine the nature of the c- erb - B -2 protein, we have now molecularly cloned complementary DNA for c- erb - B -2 messenger RNA prepared from MKN-7 cells. Its sequence shows that the c- erb - B -2 gene encodes a possible receptor protein and allows an analysis of the similarity of the protein to the EGF receptor and the neu product. As a consequence of chromosomal aberration in MKN-7 cells, a 4.6-kilobase (kb) normal transcript and a truncated 2.3-kb transcript of c- erb - B -2 are synthesized at elevated levels. The latter transcript presumably encodes only the extracellular domain of the putative receptor.

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关键词：

Operation Rapid prototyping Anatomy

DOI：

10.1038/319230a0

被引量：

3018

年份：

1986