摘要：

Liposomes with bacteriorhodopsin(bR) were prepared by mixing purple membrane and phospholipid in a supersonic bath. The blue shift of maximum absorption of bR indicated that a portin of bR molecules in vesicles are likely to be in the form of monomers. It was confirmed by the pH measurement and the flash photolysis spectroscopy that the medium was alkalized when bR vesicles were shined with light. Our experiments confirmed that bR in liposomes after self assembled in vitro are highly oriented but with an opposite preferred direction to that in the corresponding cell membrane.

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