Multistep disulfide bond formation in Yap1 is required for sensing and transduction of H2O2 stress signal.
摘要：
Redox reactions involving cysteine thiol-disulfide exchange are crucial for sensing intracellular levels of HO. However, oxidation-sensitive dithiols are also sensitive to intracellular reducing agents, and disulfide bonds are thus transient. The yeast transcription factor Yap1 is activated by disulfide-induced structural changes in the nuclear export signal in a carboxy-terminal domain. We show herein that the activation of Yap1 by HO requires multistep formation of disulfide bonds. One disulfide bond forms within 15 s in an amino-terminal domain, and then disulfide bonds linking the two domains accumulate. The multiple interdomain disulfide bonds, which result in reduction-resistant Yap1, are required for transduction of the HO stress signal to induce the appropriate level and duration of specific transcription. Our results suggest both a mechanism wherein the HO levels might be sensed by Yap1 and the way in which the NADPH levels might be maintained by altering the redox status of Yap1.
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DOI：
10.1016/j.molcel.2007.06.035
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年份：
2007
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