Unnatural amino acid incorporation into virus-like particles.
摘要：
Virus-like particles composed of hepatitis B virus (HBV) or bacteriophage Qbeta capsid proteins have been labeled with azide- or alkyne-containing unnatural amino acids by expression in a methionine auxotrophic strain of E. coli. The substitution does not affect the ability of the particles to self-assemble into icosahedral structures indistinguishable from native forms. The azide and alkyne groups were addressed by Cu(I)-catalyzed [3 + 2] cycloaddition: HBV particles were decomposed by the formation of more than 120 triazole linkages per capsid in a location-dependent manner, whereas Qbeta suffered no such instability. The marriage of these well-known techniques of sense-codon reassignment and bioorthogonal chemical coupling provides the capability to construct polyvalent particles displaying a wide variety of functional groups with near-perfect control of spacing.
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关键词：
Humans Hepatitis B virus Trypsin Capsid Proteins Amino Acids Protein Structure, Quaternary Dimerization Amino Acid Sequence Molecular Sequence Data Allolevivirus
DOI：
10.1021/bc700390r
被引量：
年份：
2008
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