A simple and rapid method for the preparation of plant genomic DNA for PCR analysis
摘要：
A method for rapid preparation of plant genomic DNA for PCR analysis was established. A small amount (4~6 mg) of leaf tissue of rice seedling, 200 滋L of TE buffer, and one tungsten bead were put into a 2 mL microcentrifuge tube. After vigorously shaking in a miller for 5 min, 1 滋L of the solution was directly applied to PCR amplification. This method is simple, rapid, high efficient, low cost, and reliable for PCR analysis, thus is es- pecially suitable for genotyping of large number of samples.
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DOI：
10.1093/nar/19.6.1349
被引量：
年份：
1991
Wiley
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