Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe.
摘要：
Homologous integration into the fission yeast Schizosaccharomyces pombe has not been well characterized. In this study, we have examined integration of plasmids carrying the leu1+ and ura4+ genes into their chromosomal loci. Genomic DNA blot analysis demonstrated that the majority of transformants have one or more copies of the plasmid vector integrated via homologous recombination with a much smaller fraction of gene conversion to leu1+ or ura4+. Non-homologous recombination events were not observed for either gene. We describe the construction of generally useful leu1+ and ura4+ plasmids for targeted integration at the leu1-32 and ura4-294 loci of S. pombe.
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关键词：
Schizosaccharomyces DNA, Fungal Chromosome Mapping Genes, Fungal Genetic Vectors Recombination, Genetic Transformation, Genetic Gene Conversion
DOI：
doi:10.1101/gad.8.5.629
被引量：
年份：
1994
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