Short-term, high-efficiency expression of transfected DNA.
摘要：
We have achieved high-efficiency uptake and expression of foreign DNA in mouse Ltk- cells by modifying the DEAE-dextran-mediated transfection method of McCutchan and Pagano (J. Natl. Cancer Inst. 42:351-357, 1968) to include an initial incubation at elevated pH followed by a shock treatment with dimethyl sulfoxide. Up to 80% of mouse Ltk- cells transfected with the herpes simplex virus thymidine kinase gene expressed thymidine kinase as measured by autoradiography.
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关键词：
Animals Mice Simplexvirus L Cells (Cell Line Transfection Kinetics Transcription, Genetic Genes Genes, Viral Thymidine Kinase
DOI：
10.1128/MCB.4.8.1641
被引量：
年份：
1984
Semantic Scholar
万方医学
掌桥科研
NCBI
Europe PMC
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