Molecular cloning and expression of the fas ligand, a novel member of the tumor necrosis factor family

来自 Elsevier

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阅读量：

241

作者：

Takashi Suda ★，Tomohiro Takahashi ★，P Golstein，Shigekazu Nagata ★

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摘要：

The Fas antigen (Fas) belongs to the tumor necrosis factor (TNF)/nerve growth factor receptor family, and it mediates apoptosis. Using a soluble form of mouse Fas, prepared by fusion with human immunoglobulin Fc, Fas ligand was detected on the cell surface of a cytotoxic T cell hybridoma, PC60-d10S. A cell population that highly expresses Fas ligand was sorted using a fluorescence-activated cell sorter, and its cDNA was isolated from the sorted cells by expression cloning. The amino acid sequence indicated that Fas ligand is a type II transmembrane protein that belongs to the TNF family. The recombinant Fas ligand expressed in COS cells induced apoptosis in Fas-expressing target cells. Northern hybridization revealed that Fas ligand is expressed in activated splenocytes and thymocytes, consistent with its involvement in T cell-mediated cytotoxicity and in several nonlymphoid tissues, such as testis.

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关键词：

ATP SYNTHASE 2-DIMENSIONAL CRYSTAL ELECTRON MICROSCOPY IMAGE PROCESSING

DOI：

10.1016/0092-8674(93)90326-L

被引量：

6447

年份：

1993