Selective detection of phosphotyrosine in the presence of various phosphate-containing biomolecules with the aid of a terbium(III) complex.
摘要:
Finding phosphotyrosine: Phosphotyrosine (either in its monomeric form or as a component of oligopeptides) was selectively detected by using luminescence in the presence of a specialized terbium(III) complex, without any signals from nonphosphorylated tyrosine, phosphoserine, phosphothreonine, nucleotides, or nucleic acids. Tyrosine phosphorylation could clearly be distinguished even in the presence of single-stranded RNA.
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DOI:
10.1002/cbic.200900227
被引量:
年份:
2010
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