Asymmetric synthesis of (R)-1,3-butanediol from 4-hydroxy-2-butanone by a newly isolated strain Candida krusei ZJB-09162.

阅读量:

121

作者:

RC ZhengZ GeZK QiuYS WangYG Zheng

展开

摘要:

1,3-butanediol has been attracting much attention in pharmaceuticals industry. A new ideal strain, ZJB-09162, which exhibited high reduction activity and excellent ()-stereospecificity towards 4-hydroxy-2-butanone, has been successfully isolated from soil samples. Based on morphology, physiological tests (API 20C AUX), and 5.8S-ITS sequence, the isolate was identified as )-1,3-Butanediol was produced in 19.8g/L, 96.6% conversion, and 99.0% at optimal pH 8.5, 35°C with a 2:1 molar ratio of glucose to 4H2B. In order to achieve higher product titer, the substrate loading was optimized in fixed catalysts and fixed substrate/catalysts ratio mode. The bioreduction of 4-hydroxy-2-butanone at a concentration of 45.0g/L gave ()-1,3-butanediol in 38.7g/L and 83.9% conversion. Therefore, )-1,3-butanediol.

展开

DOI:

10.1007/s00253-012-3942-2

被引量:

9

年份:

2012

通过文献互助平台发起求助,成功后即可免费获取论文全文。

我们已与文献出版商建立了直接购买合作。

你可以通过身份认证进行实名认证,认证成功后本次下载的费用将由您所在的图书馆支付

您可以直接购买此文献,1~5分钟即可下载全文,部分资源由于网络原因可能需要更长时间,请您耐心等待哦~

身份认证 全文购买

相似文献

参考文献

引证文献

引用走势

2014
被引量:4

辅助模式

0

引用

文献可以批量引用啦~
欢迎点我试用!

引用