Asymmetric synthesis of (R)-1,3-butanediol from 4-hydroxy-2-butanone by a newly isolated strain Candida krusei ZJB-09162.

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121

作者：

RC Zheng，Z Ge，ZK Qiu，YS Wang，YG Zheng

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摘要：

1,3-butanediol has been attracting much attention in pharmaceuticals industry. A new ideal strain, ZJB-09162, which exhibited high reduction activity and excellent ()-stereospecificity towards 4-hydroxy-2-butanone, has been successfully isolated from soil samples. Based on morphology, physiological tests (API 20C AUX), and 5.8S-ITS sequence, the isolate was identified as )-1,3-Butanediol was produced in 19.8g/L, 96.6% conversion, and 99.0% at optimal pH 8.5, 35°C with a 2:1 molar ratio of glucose to 4H2B. In order to achieve higher product titer, the substrate loading was optimized in fixed catalysts and fixed substrate/catalysts ratio mode. The bioreduction of 4-hydroxy-2-butanone at a concentration of 45.0g/L gave ()-1,3-butanediol in 38.7g/L and 83.9% conversion. Therefore, )-1,3-butanediol.

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关键词：

R)-1 3-Butanediol Candida krusei ZJB-09162 Carbonyl reductase Asymmetric reduction NADH-dependent

DOI：

10.1007/s00253-012-3942-2

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年份：

2012