Quantitative isolation of total glycosphingolipids from animal cells
摘要：
The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described. The method consists of (a) acetylation of total lipids with pyridine and acetic anhydride, (b) separation of acetylated glycolipids from nonglycolipids on a magnesia-silica gel (Florisil) column, and (c) deacetylation of glycolipid in chloroform-methanol-sodium methoxide. This method is useful for determination of microgram quantities of glycolipids derived from less than 1 ml of packed cells.
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关键词：
Animals Rats Spleen Liver Kidney Fibroblasts Carcinoma, Hepatocellular Liver Neoplasms Methanol Acetone
DOI：
doi:10.1016/0020-711X(71)90065-6
被引量：
年份：
1971
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