Targeted disruption of chain membrane exon causes loss of heavy-chain allelic exclusion

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摘要：

BURNET'S clonal selection theorysuggests that each B lymphocyte is committed to a single antibody specificity. This is achieved by a programme of somatic rearrangements of the gene segments encoding antibody variable (V) regions, in the course of B-cell development. Evidence from immunoglobulin-transgenic mice and immunoglobulin-gene-transfected transformed pre-B cells suggests that the membrane form of the immunoglobulin heavy (H) chain of class(m), expressed from a rearranged H-chain (IgH) locus, may signal allelic exclusion of the homologous IgH locus in the celland initiation of light (L)-chain gene rearrangement in the Ig/c loci6. We report here that targeted disruption of the membrane exon of thechain indeed results in the loss of H-chain allelic exclusion. But, some K chain gene rearrangement is still observed in the absence ofm expression.

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Animals Mice, Inbred BALB C Mice, Inbred C57BL Mice Spleen B-Lymphocytes Bone Marrow Cells DNA Polymerase Chain Reaction Base Sequence