Transposition of R factor genes to bacteriophage lambda.
摘要：
Transpositions of segments of R factors (antibiotic resistance plasmids) to bacteriophage λ have been selected and characterized. Cells of Escherichia coli harboring R factors that determine kanamycin resistance were infected with phage λ , and λ kan transducing lines were obtained. Each of the three examined is unusual when compared to λ transducing phages containing E. coli chromosomal genes: the kan insertions (a) occur at several sites, each well removed from the integration region POP′, (b) are not associated with deletion of λ phage DNA, and (c) are separable from the λ genome during transduction or during lytic growth. Two insertions from the same R factor contain 1.5 kilobase sequences repeated in inverted order. The properties of the λ kan phages suggest that R factors contain systems capable of mediating genetic exchange in the absence of extensive DNA homology. It is suggested that such systems of exchange may have played important roles in R factor evolution.
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关键词：
Genetics R factor evolution Inverted repetition Kanamycin resistance Heteroduplex mapping Restriction endonuclease
DOI：
10.1073/pnas.72.9.3628
被引量：
年份：
1975
掌桥科研
万方医学
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