摘要：

In enzymatic amplification systems for detecting mediators, laccase (EC 1.10.3.2) in a dissolved or immobilized form is brought together as mediator-oxidizing enzyme with a mediator-reducing enzyme in front of an oxygen electrode. These systems are characterized in that p-aminophenol is present as mediator and oligosaccharide dehydrogenase or NADH-independent glucose dehydrogenase (EC 1.1.99.17) is present as mediator-reducing enzyme. These new enzymatic amplification systems may be used for very sensitive p-aminophenol detection assays and for enzyme immunoassays.

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