摘要：

Nucleoside oxidase, a novel nucleoside oxidizing enzyme has been purified from a crude extract of Pseudomonas maltophilia LB-86 by a ammonium sulfate fractionation, heat treatment, column chromatography on DEAE-Toyopearl, and gel filtration twice on Sephacryl S-200. The overall purification was approximately 60-fold with a yield of 35%. The purified enzyme gave a single protein band on acrylamide gel electrophoresis. Reaction products from inosine were identified as inosine-5'-aldehyde and inosine-5'-carboxylic acid. The enzyme catalyzed the oxidation of inosine to inosine-5'-carboxylic acid via inosine-5'-aldehyde using molecular oxygen as a primary electron acceptor with no formation of hydrogen peroxide.

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