Histone acetylation: influence on transcription, nucleosome mobility and positioning, and linker histone‐dependent transcriptional repression
摘要：
We demonstrate using a dinucleosome template that acetylation of the core histones enhances transcription by RNA polymerase III. This effect is not dependent on an increased mobility of the core histone octamer with respect to DNA sequence. When linker histone is subsequently bound, we find both a reduction in nucleosome mobility and a repression of transcription. These effects of linker histone binding are independent of core histone acetylation, indicating that core histone acetylation does not prevent linker histone binding and the concomitant transcriptional repression. These studies are complemented by the use of a egg extract competent both for chromatin assembly on replicating DNA and for RNA polymerase III transcription. Incorporation of acetylated histones and lack of linker histones together facilitate transcription by >10-fold in this system; however, they have little independent effect on transcription. Thus core histone acetylation significantly facilitates transcription, but this effect is inhibited by the assembly of linker histones into chromatin.
展开
DOI：
10.1093/emboj/16.8.2096
被引量：
年份：
1997
Nature
Wiley
EBSCO
Wiley
(全网免费下载)
万方医学
查看更多
Ingenta
Europe PMC
Europe PMC
(全网免费下载)
NCBI
(全网免费下载)
Cell Press
NCBI
NCBI
AACR
JBC
NRC Research Press
ResearchGate
ResearchGate
(全网免费下载)
ProQuest
EBSCO
waseda.pure.elsevier.com
bloodjournal.org
hw-f5-embojnl.highwire.org
onAcademic
embojnl.embopress.org
(全网免费下载)
199.171.202.195
deepdyve.com
mendeley.com
dev.biologists.org
core.ac.uk
circres.ahajournals.org
jvi.asm.org
emboj.embopress.org
(全网免费下载)
jimmunol.org
jem.rupress.org
emboj.embopress.org
elifesciences.org
bvsalud.org
jme.endocrinology-journals.org
pnas.org
mysciencework.com
scienceopen.com
embopress.org
(全网免费下载)
Wiley
(全网免费下载)
Europe PMC
(全网免费下载)
NCBI
(全网免费下载)
ResearchGate
(全网免费下载)
embojnl.embopress.org
(全网免费下载)
embopress.org
(全网免费下载)
emboj.embopress.org
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
