摘要：

Astrocytes can respond to a variety of stimuli by elevating their cytoplasmic Ca²⁺ concentration and can in turn release glutamate to signal adjacent neurons. The majority of this Ca²⁺ is derived from internal stores while a portion also comes from outside of the cell. Astrocytes use Ca²⁺ entry through store-operated Ca²⁺ channels to refill their internal stores. Therefore, we investigated what role this store-operated Ca²⁺ entry plays in astrocytic Ca²⁺ responses and subsequent glutamate release. Astrocytes express canonical transient receptor potential (TRPC) channels that have been implicated in mediating store-operated Ca²⁺ entry. Here, we show that astrocytes in culture and freshly isolated astrocytes from visual cortex express TRPC1, TRPC4, and TRPC5. Indirect immunocytochemistry reveals that these proteins are present throughout the cell; the predominant expression of functionally tested TRPC1, however

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