Thiazolidinediones repress ob gene expression in rodents via activation of peroxisome proliferator-activated receptor gamma.
摘要：
The ob gene product, leptin, is a signaling factor regulating body weight and energy balance. ob gene expression in rodents is increased in obesity and is regulated by feeding patterns and hormones, such as insulin and glucocorticoids. In humans with gross obesity, ob mRNA levels are higher, but other modulators of human ob expression are unknown. In view of the importance of peroxisome proliferator-activated receptor gamma (PPARgamma) in adipocyte differentiation, we analyzed whether ob gene expression is subject to regulation by factors activating PPARs. Treatment of rats with the PPARalpha activator fenofibrate did not change adipose tissue and body weight and had no significant effect on ob mRNA levels. However, administration of the thiazolidinedione BRL49653, a PPARgamma ligand, increased food intake and adipose tissue weight while reducing ob mRNA levels in rats in a dose-dependent manner. The inhibitory action of the thiazolidinedione BRL49653 on ob mRNA levels was also observed in vitro. Thiazolidinediones reduced the expression of the human ob promoter in primary adipocytes, however, in undifferentiated 3T3-L1 preadipocytes lacking endogenous PPARgamma, cotransfection of PPARgamma was required to observe the decrease. In conclusion, these data suggest that PPARgamma activators reduce ob mRNA levels through an effect of PPARgamma on the ob promoter.
展开
关键词：
Animals Humans Rats Adipose Tissue Liver Thiazoles Thiazolidinediones Leptin Transcription Factors DNA Primers
DOI：
10.1172/JCI118860
被引量：
年份：
1996
国家科技图书文献中心
(权威机构)
掌桥科研
万方医学
dx.doi.org
BioOne
查看更多
Oxford Univ Press
NCBI
Europe PMC
NCBI
(全网免费下载)
NCBI
Pubmed Central
(全网免费下载)
Europe PMC
(全网免费下载)
ResearchGate
ResearchGate
(全网免费下载)
ProQuest
Citeseer
(全网免费下载)
diabetes.diabetesjournals.org
dm5migu4zj3pb.cloudfront.net
joe.endocrinology-journals.org
jn.nutrition.org
jacionline.org
jnm.snmjournals.org
jci.org
(全网免费下载)
content-assets.jci.org
(全网免费下载)
rstb.royalsocietypublishing.org
cghjournal.org
mendeley.com
core.ac.uk
学术范
(全网免费下载)
fasebj.org
scindeks.ceon.rs
mysciencework.com
infoscience.epfl.ch
ajpregu.physiology.org
jci.org
molpharm.aspetjournals.org
atvb.ahajournals.org
jleukbio.org
学术范
infoscience.epfl.ch
(全网免费下载)
jlr.org
Europe PMC
(全网免费下载)
NCBI
(全网免费下载)
Pubmed Central
(全网免费下载)
ResearchGate
(全网免费下载)
Citeseer
(全网免费下载)
content-assets.jci.org
(全网免费下载)
jci.org
(全网免费下载)
infoscience.epfl.ch
(全网免费下载)
学术范
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
