摘要：

Objective To observe the effects of compound ketamine oral solution(CKOS)on rats gastric NMDAR1 and GABAA receptors in order to find out the effects of CKOS on gastrointestinal motility,and evaluate the security of CKOS.Methods Fifty male healthy Sprague-Dawley rats were randomly divided into 10 time points.Each time point had 5 rats.Rats in 0 min time point were irrigated with 0.9%NS 2 μL/g for contrast.Others were irrigated with CKOS 2 μL/g(KET 100 mg/kg,MID 10 mg/kg).After drug administration respectively at the time of 0,5,10,15,30,60,90,120,240,360 min,catheter was inserted from right heart to irrigate 0.9% cold NS,the colour of liver changed from red to white(150 mL),extra fluid were removed from venecava.Then after all body organs were fixed with 4% polymaldehyde,stomach were taken out and put into 4 ℃ refrigerator,the changes of NMDAR1,GABAAR in rats stomach were observed by immune histochemistry staining and in situ hybridization.Results The NMDAR1 expression began to decrease at 10 min after taking CKOS,and decreased significantly between 15~120 min,recovered at 240 min,but was still lower than control group.At 360 min,it was still unrecovered.The GABAAR expression began to increase at 30 min after taking CKOS,increased significantly at 60～90 min. Recovery began at 240 min,and it recovered to normal at 360 min.Conclusion CKOS can inhibit the expression of NMDAR1 and increase the expression of GABAAR in rats′ stomach,which may be one of the reasons for CKOS slowering gastric fluid emptying in rats.

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