Protein "fingerprinting" in complex mixtures with peptoid microarrays
摘要：
We report here that microarrays comprised of several thousand peptoids (oligo-N-substituted glycines) are useful tools for the identification of proteins via a "fingerprinting" approach. By using maltose-binding protein, glutathione S-transferase, and ubiquitin, a specific and highly reproducible pattern of binding was observed when fluorescently labeled protein was hybridized to the array. A similar pattern was obtained when binding of an unlabeled protein to the array was visualized by secondary hybridization of a labeled antibody against that protein, showing that native proteins can be identified without the requirement for prior chemical labeling. This work suggests that small-molecule microarrays might be used for more complex fingerprinting assays of potential diagnostic value.
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关键词：
profiling proteomics small molecule microarrays SMALL-MOLECULE MICROARRAYS DIVERSITY-ORIENTED SYNTHESIS STOCK SOLUTION APPROACH SOLID-PHASE SYNTHESIS PEPTIDE ARRAY PHOTOLITHOGRAPHIC SYNTHESIS COMBINATORIAL CHEMISTRY CHEMICAL GENETICS ONE-BEAD DISCOVERY
DOI：
10.1073/pnas.0501208102
被引量：
年份：
2005
国家科技图书文献中心
(权威机构)
Wiley
万方医学
掌桥科研
dx.doi.org
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