Enhancing viability of two biocontrol yeasts in liquid formulation by applying sugar protectant combined with antioxidant

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摘要：

Survivals of Cryptococcus laurentii and Pichia membranaefaciens in liquid formulations with sugar protectants (trehalose and galactose) and L -ascorbic acid (Vc) were investigated during storage at 4°C and 25°C. When galactose or trehalose was used alone as protectant, C. laurentii maintained relatively high viability in potassium phosphate buffer. Addition of Vc to trehalose improved its protective effect. P. membranaefaciens maintained viability >60% after 90days at 4°C when 5% galactose served as a protectant, and its combination with Vc was the most effective at maintaining viability. Moreover, liquid formulation kept higher viability of the two yeasts at 4°C than at 25°C. Biocontrol efficiency of the two yeasts was maintained after formulation and storage. The results indicate that trehalose is considered as a suitable protectant for liquid formulation of C. laurentii , while galactose is better for P. membranaefaciens . Combining Vc with the sugars improves the protective efficiency.

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关键词：

Viability Biocontrol yeasts Liquid formulation Sugar protectant Antioxidant

DOI：

10.1007/s10526-009-9229-6

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年份：

2009