登录注册
高级搜索
包含全部检索词
包含精确检索词
包含至少一个检索词
不包含检索词
出现检索词的位置
文章任何位置
位于文章标题
作者
机构
出版物 期刊
会议
发表时间  - 
语言检索范围 不限
不限 英文 中文
文献
期刊
学者
论文查重 优惠
论文查重
开题分析
单篇购买
文献互助

Biotinyl and phosphotyrosinyl phosphoramidite derivatives useful in the incorporation of multiple reporter groups on synthetic oligonucleotides.

来自 Semantic Scholar

阅读量：

72

作者：

M Konrad，D Ian，MR Evans，MJ Gait

展开

摘要：

Non-nucleosidic phosphoramidite linker units suitable for use on commercial DNA synthesis machines have been designed for the direct incorporation of biotin and a new reporter group, phosphotyrosine, at multiple sites on synthetic oligonucleotides. The units are based on a 3-carbon glyceryl backbone where the reporter group is attached to the 2-O-position through a 3-aminopropyl spacer. 17-mer oligonucleotides were synthesized carrying at the 5'-end 1, 2, 4 or 8 biotinyl units or 1, 2, 4 or 8 phosphotyrosinyl units respectively and used for the detection of DNA on nitrocellulose filters by hybridization. Subsequent incubation of the filters with a monoclonal antibody to the reporter group followed by secondary detection using enhanced chemiluminescence (ECL) resulted in amplification of signal strengths as the number of reporter groups was increased. The results were quantitated by use of a charge couple device (CCD) camera. Spacing of biotin moieties by thymidyl residues resulted in further improvements in signal strengths, whereas similar spacing of phosphotyrosinyl units did not.

展开

关键词：

Tyrosine Phosphotyrosine Oligodeoxyribonucleotides Electrophoresis, Polyacrylamide Gel Base Sequence Molecular Structure Molecular Sequence Data Biotin Organophosphorus Compounds Chromatography, High Pressure Liquid

DOI：

10.1093/nar/18.15.4345

被引量：

162

年份：

1990

收藏 引用 批量引用 分享
全部来源 免费下载 全文购买

通过文献互助平台发起求助，成功后即可免费获取论文全文。

我们已与文献出版商建立了直接购买合作。

你可以通过身份认证进行实名认证，认证成功后本次下载的费用将由您所在的图书馆支付

您可以直接购买此文献，1~5分钟即可下载全文，部分资源由于网络原因可能需要更长时间，请您耐心等待哦~

身份认证 全文购买

相似文献

参考文献

引证文献

来源期刊

Nucleic Acids Research

研究点推荐

引用走势

1993
被引量：21

辅助模式

0

引用

文献可以批量引用啦~
欢迎点我试用！

引用