Modulation of transcription factor NF-kappa B binding activity by oxidation-reduction in vitro.
摘要：
NF-κ B is a widely used regulator of inducible and tissue-specific gene control. In the cytosol, when complexed to an inhibitory molecule, Iκ B, NF-κ B is in an inactive form and cannot bind DNA. Activation of cells with appropriate stimuli results in the dissociation of NF-κ B from Iκ B and its translocation to the nucleus as an active binding protein. We now demonstrate that NF-κ B binding in vitro can be inhibited by agents that modify free sulfhydryls. Binding is eliminated after treatment with N-ethylmaleimide, an alkylating agent, and diamide, an oxidizing agent. The diamide effect can be reversed by 2-mercaptoethanol. Further, 2-mercaptoethanol acts synergistically with deoxycholate plus Nonidet P-40 in converting inactive cytosolic NF-κ B to an active DNA-binding form. It is therefore possible that modulation of the redox state of NF-κ B could represent a post-translational control mechanism for this factor.
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DOI：
10.1073/pnas.88.10.4328
被引量：
年份：
1991
Taylor & Francis
BMJ
掌桥科研
万方医学
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