Cloning of bcl-6, the Locus Involved in Chromosome Translocations Affecting Band 3q27 in B-Cell Lymphoma

来自 NCBI

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作者：

BH Ye，PH Rao，RSK Chaganti，R Dalla-Favera

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摘要：

Chromosomal translocations involving band 3q27 and various chromosomal sites, including the sites of the immunoglobulin (Ig) loci (14q32, 2p12, 22q11), represent recurrent aberrations in (). In order to identify the putative protooncogene involved in these translocations, we have cloned the breakpoints from two B-cases carrying t(3;14)(q27;q32) translocations by screening genomic DNA libraries constructed from biopsy samples with immunoglobulin probes. Several recombinant phages have been obtained from each case and shown to contain sequences from both 14q32 and 3q27 by fluorescence in situ hybridization mapping on . In both cases, the translocation breakpoints were found within the switch region of the Ig heavy-chain locus on 14q32 and within the same 3-kilobase region on 3q27. When used in Southern blot hybridization, a probe from the 3q27 region detected rearrangements in an additional five cases carrying 3q27 translocations with 14q32 or other genomic sites. The same probe detected a predominant 2.4-kilobase mRNA species in several lymphoid lines analyzed by Northern blot hybridization. These data suggest that chromosomal breakpoints in 3q27 cluster in the proximity of a transcribed gene which represents a candidate protooncogene () involved in B-.

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关键词：

HYPERTHERMIA PROTEIN-B23 SILVER STAINING NUCLEOLAR PROTEINS

DOI：

doi:10.1016/0304-3835(93)90085-N

被引量：

983

年份：

1993