摘要：

During the perinatal period, oligodendroglial precursor proliferate rapidly, then cease dividing and differentiate into oligodendroglia. Many of these newly formed oligodendroglia are destined to die. We now demonstrate that oligodendroglia generated in passaged cultures of forebrain oligodendroglial precursor after removal of (basic ) from the medium often undergo internucleosomal DNA nicking and nuclear fragmentation, features characteristic of apoptosis. These alterations are rare in cultures maintained continuously in basic . As in many other cellular lineages susceptible to apoptosis, these degenerative changes can be prevented by treatment with the antagonist, aurintricarboxylic acid, or by inhibiting de novo RNA or . Supplementation of the basic -free medium with , -1, platelet-derived , or ciliary neuronotrophic also diminishes DNA nicking. Both oligodendroglial differentiation and DNA nicking are induced in basic -treated cultures by inhibiting DNA with aphidicholin or excess , thus suggesting a close linkage between the anti-apoptotic, anti-differentiation, and mitogenic effects of basic on the oligodendroglial lineage.

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